Pairing levels by FISH for sgs1Δ ndt80Δ
| Pairing levela | |||||
|---|---|---|---|---|---|
| Genotype | Experiment | VIIIobs; XIobs; bkgd | VIIIobs–bkgd; XIobs–bkgd | n | % wild type |
| ndt80Δ | 1 | 0.70; 0.78; 0.03 | 0.67; 0.75 | 93 | |
| ndt80Δ | 2 | 0.83; 0.88; 0.03 | 0.80; 0.85 | 105 | |
| Average (VIII; XI) | 0.74 ± 0.09; 0.80 ± 0.07 | 100 | |||
| sgs1Δ ndt80Δ | 1 | 0.65; 0.57; 0.07 | 0.58; 0.50 | 77 | |
| sgs1Δ ndt80Δ | 2 | 0.59; 0.72; 0.07 | 0.52; 0.65 | 92 | |
| Average (VIII; XI) | 0.55 ± 0.04; 0.58 ± 0.11 | 73 | |||
↵a Pairing assessed at t = 6 hr in SPM with fluorescently labeled cosmid probes hybridized to spread nuclei preparations. Observed pairing levels are the fraction of nuclei scored with a distance between foci of ≤0.7 μm. Background pairing levels for each hybridization were determined as the fraction of nuclei scored with nonhomologous pairs of loci that were ≤0.7 μm apart.