TABLE 1

Pairing levels by FISH for sgs1Δ ndt80Δ

Pairing levela
GenotypeExperimentVIIIobs; XIobs; bkgdVIIIobs–bkgd; XIobs–bkgdn% wild type
ndt80Δ10.70; 0.78; 0.030.67; 0.7593
ndt80Δ20.83; 0.88; 0.030.80; 0.85105
Average (VIII; XI)0.74 ± 0.09; 0.80 ± 0.07100
sgs1Δ ndt80Δ10.65; 0.57; 0.070.58; 0.5077
sgs1Δ ndt80Δ20.59; 0.72; 0.070.52; 0.6592
Average (VIII; XI)0.55 ± 0.04; 0.58 ± 0.1173
  • a Pairing assessed at t = 6 hr in SPM with fluorescently labeled cosmid probes hybridized to spread nuclei preparations. Observed pairing levels are the fraction of nuclei scored with a distance between foci of ≤0.7 μm. Background pairing levels for each hybridization were determined as the fraction of nuclei scored with nonhomologous pairs of loci that were ≤0.7 μm apart.