TABLE 2

Detecting RMCE using negative selection

Genomic positionDonor cassetteEmbryos injectedLarvae hatchedFertile adultsVials with RMCEaVials with RMCE (%)
25CGFP5002721741910.9
42AGFP330147922223.9
  • Putative RMCE events were detected only by the loss of the mini-white marker phenotype.

  • a In addition to vials producing flies resulting from RMCE, 10 vials produced flies with increased eye pigmentation, which were not analyzed further.