TABLE 1

RMCE in Drosophila using ϕC31 integrase

Genomic positionDonor cassetteEmbryos injectedLarvae hatchedFertile adultsw y+ (RMCE)aw+ y+(intermediate)bVials with RMCE (%)
25Cyellow38019369507.2
42Ayellow520333106312.8
52Dyellow48025490818.8
82Fyellow44020494656.4
  • Parental lines each contain a single P-element insertion carrying the mini-white gene. RMCE events were detected by screening for flies with w eyes and y+ pigmented cuticle, indicating loss of mini-white and gain of yellow, respectively. Single crossover events retained mini-white and gained yellow. In parental line 42A, we see evidence of variegation in mini-white expression.

  • a Number of vials containing flies resulting from RMCE.

  • b Number of vials containing flies resulting from single crossovers. Three of these seven vials also produced flies resulting from RMCE.