Summary of synMuv genetic interactions

Phenotype of double mutant with mutation of specified classPhenotype of double mutant with let-23Inferred synMuv class of mutant gene
MutationClass AClass BClass Cgap-1sli-1ark-1
lin-15A(n767) lin-38(n751)non-MuvMuvMuvMuvMuvMuvVulA
lin-15B(n744) lin-35(n745) lin-65(n3441) mep-1(n3703)aMuvNon-MuvMuvNon-MuvNon-MuvNon-MuvVulB
lin(n3628)MuvNon-MuvNon-MuvNon-MuvNon-MuvNon-MuvVulB or lin(n3628)b
  • We provisionally assign 29 genes to six synMuv classes. The assignments of 11 of these genes (shown above and underlined below) are based on extensive genetic interaction tests. The remaining 18 genes have not been tested as extensively. However, on the basis of known genetic interactions and molecular identities, we speculate that most of these 18 genes will remain in the classes to which they have previously been assigned.

    • Class A: lin-8, lin-15A, lin-38, lin-56.

    • Class B: lin-9, lin-13, lin-15B, lin-35, lin-36, lin-37, lin-52, lin-53, lin-54, lin-61, lin-65, dpl-1, efl-1, hda-1, hpl-2, let-418, lin(n3628), mep-1.

    • Class C: trr-1, mys-1, epc-1, ssl-1.

    • gap-1: gap-1.

    • sli-1: sli-1.

    • ark-1: ark-1.

  • NA, not applicable; since each of these classes contains only one gene, double mutants within the same class cannot be constructed.

  • a mep-1(n3703) and class C synMuv mutations interact to cause larval lethality at a stage earlier than vulval abnormalities can be determined.

  • b Like class B synMuv mutations, lin(n3628) interacts synthetically with class A mutations; does not interact synthetically with class B, ark-1, gap-1, or sli-1 mutations; and does not suppress the Vul phenotype of let-23(sy97). However, lin(n3628) does not interact synthetically with class C mutations. lin(n3628) may define yet another class of synMuv genes. Alternatively, the mutation n3628 may be a partial loss-of-function mutation too weak to reveal redundancy with class C genes, in which case lin(n3628) may be a class B gene. Determination of the lin(n3628) null phenotype and genetic interaction tests with a null mutation of this gene should distinguish between these possibilities.