TABLE 2

Three- and four-factor crosses

GeneGenotype of heterozygotePhenotype of selected recombinantsGenotype of selected recombinants (with respect to unselected markers)
lin-65+ lin-65 +/bli-3 + lin-17; lin-15A(n767)Lin-179/19 lin-65/+
bli-3 + lin-65/+ spe-15 +; lin-15A(n767)Muv10/18 spe-15/+
+ lin-65 lin-17/spe-15 + +; lin-15A(n767)Lin-1711/11 spe-15/+
bli-3 + + + lin-65 +/+ Y73E7.2 Y71G12B.2 Y71G12B.17 + Y71G12B.18; lin-15A(n767)Muv4/30 Y73E7.2/+
Muv2/30 Y71G12B.2/+
Muv1/30 Y71G12B.17/+
Muv0/30 Y71G12B.18/+
+ lin-65 + + + lin-17/Y71G12B.17 + Y71G12B.18 Y71G12B.27 M01D7.2 +; lin-15A(n767)Lin-1717/23 M01D7.2/+
Lin-1718/23 Y71G12B.27/+
Lin-1721/23 Y71G12B.18/+
Lin-1723/23 Y71G12B.17/+
lin(n3542)+ + lin(n3542) lin-15A(n767)/unc-10 dpy-6 + lin-15A(n767)Unc8/8 lin(n3542)/+
+ lin(n3542) + lin-15A(n767)/dpy-6 + unc-9 lin-15A(n767)Unc4/40 lin(n3542)/+
lin(n3628)lin(n3628) + +/+ dpy-5 unc-13; lin-15A(n767)Dpy0/6 lin(n3628)/+
Unc6/6 lin(n3628)/+
+ lin(n3628) +/unc-11 + dpy-5; lin-15A(n767)Unc1/11 lin(n3628)/+
Dpy5/11 lin(n3628)/+
unc-11 + + lin(n3628)/+ unc-73 lin-44 +; lin-15A(n767)Muv3/9 unc-73 lin-44/++
+ + lin(n3628) dpy-5/unc-73 lin-44 + +; lin-15A(n767)Muv0/21 unc-73 lin-44/++
lin(n3628) + dpy-5/+ unc-38 +; lin-15A(n767)Muv3/7 unc-38/+
unc-11 lin(n3628) +/+ + unc-38; lin-15A(n767)Muv0/9 unc-38/+
mep-1+ mep-1 +/unc-5 + dpy-20; lin-15A(n767)Unc56/57 mep-1/+
Dpy2/61 mep-1/+
mep-1 + +/+ dpy-20 unc-30; lin-15A(n767)Dpy0/51 mep-1/+
Unc58/58 mep-1/+
+ + mep-1 +/unc-24 mec-3 + dpy-20; lin-15A(n767)Unc Mec10/12 mep-1/+
Unc17/17 mep-1/+
Mec Dpy0/8 mep-1/+
Dpy2/8 mep-1/+
+ mep-1 dpy-20 +/lin-3 + + unc-22; lin-15A(n767)Dpy5/5 lin-3/+
Vul3/10 mep-1/+
+ + mep-1+/mec-3 sem-3 + dpy-20; lin-15A(n767)Mec17/17 mep-1/+
Dpy6/13 mep-1/+
mys-1+ mys-1 +/unc-46 + dpy-11; lin-15AUnc3/7 mys-1/+
Dpy7/11 mys-1/+
trr-1+ rol-6 + trr-1/dpy-10 + unc-4 +; lin-15A(n767)Rol3/14 unc-4/+
Dpy3/3 trr-1/+
Unc0/8 trr-1/+
+ trr-1 +/dpy-10 + rol-1; lin-15A(n767)Rol9/20 trr-1/+
+ + trr-1/dpy-10 unc-53 +; lin-15A(n767)Unc0/17 trr-1/+
+ trr-1 +/unc-53 + rol-1; lin-15A(n767)Unc7/10 trr-1/+
Rol7/10 trr-1/+
+ trr-1 + rol-1/unc-4 + mex-1 +; lin-15A(n767)Rol12/14 mex-1/+
  • Three- and four-factor crosses were performed using standard methods (Brenner 1974). We mapped lin-65 using the Y73E7.2, Y71G12B.2, Y71G12B.17, Y71G12B.18, Y71G12B.27, M01D7.2 DNA sequence polymorphisms present in the CB4856 strain.