TABLE 1

Phenotypes of synMuv mutant strains

% Muv (n)
Genotype15°20°Additional abnormalities
ark-1(n3524); lin-15A(n767)0 (251)80 (171)
ark-1(n3701); lin-15A(n767)12 (190)95 (160)
dpl-1(n3643); lin-15A(n767)a99 (154)100 (252)
efl-1(n3639); lin-15A(n767)a93 (74)100 (78)Ste
gap-1(n3535) lin-15A(n767)1 (143)50 (236)
let-418(n3536); lin-15A(n767)0 (201)55 (183)hs Ste
let-418(n3626); lin-15A(n767)2 (62)97 (76)Ste
let-418(n3629); lin-15A(n767)0 (52)86 (58)Ste
let-418(n3634); lin-15A(n767)0 (87)92 (48)Ste
let-418(n3635); lin-15A(n767)0 (76)71 (70)Ste
let-418(n3636); lin-15A(n767)0 (77)92 (78)Ste
let-418(n3719); lin-15A(n767)0 (101)100 (60)Ste
lin-9(n3631); lin-15A(n767)100 (42)100 (72)Ste
lin-9(n3675); lin-15A(n767)43 (166)100 (105)
lin-9(n3767); lin-15A(n767)100 (67)100 (56)Ste
lin-13(n3642); lin-15A(n767)3 (60)100 (63)Ste
lin-13(n3673); lin-15A(n767)61 (145)97 (129)
lin-13(n3674); lin-15A(n767)78 (131)100 (191)hs Ste
lin-13(n3726); lin-15A(n767)31 (225)99 (149)hs Ste
lin-15B(n3436) lin-15A(n767)100 (193)100 (212)
lin-15B(n3676) lin-15A(n767)18 (167)72 (130)
lin-15B(n3677) lin-15A(n767)99 (111)100 (122)
lin-15B(n3711) lin-15A(n767)100 (186)100 (156)
lin-15B(n3760) lin-15A(n767)32 (171)100 (150)
lin-15B(n3762) lin-15A(n767)63 (113)97 (116)
lin-15B(n3764) lin-15A(n767)96 (232)100 (199)
lin-15B(n3766) lin-15A(n767)55 (132)100 (173)
lin-15B(n3768) lin-15A(n767)80 (159)100 (302)
lin-15B(n3772) lin-15A(n767)100 (220)100 (191)
lin-35(n3438); lin-15A(n767)100 (153)100 (126)Partial Ste at 20°, Rup
lin-35(n3763); lin-15A(n767)100 (108)100 (160)Partial Ste at 20°, Rup
lin-36(n3671); lin-15A(n767)65 (191)100 (151)
lin-36(n3672); lin-15A(n767)98 (198)100 (178)
lin-36(n3765); lin-15A(n767)0 (184)37 (202)
lin-52(n3718); lin-15A(n767)b100 (41)100 (82)Ste
lin-53(n3448); lin-15A(n767)67 (130)100 (211)Partial Ste at 20°
lin-53(n3521); lin-15A(n767)100 (34)100 (125)Partial Ste at 20°
lin-53(n3622); lin-15A(n767)85 (61)100 (66)Ste
lin-53(n3623); lin-15A(n767)24 (55)100 (51)Ste
lin-65(n3441); lin-15A(n767)80 (165)99 (195)
lin-65(n3541); lin-15A(n767)79 (242)98 (137)
lin-65(n3543); lin-15A(n767)85 (177)100 (121)
lin(n3628); lin-15A(n767)3 (103)84 (188)
lin(n3542) lin-15A(n767)0 (127)35 (218)
mep-1(n3680); lin-15A(n767)5 (122)97 (105)hs Ste
mep-1(n3702); lin-15A(n767)30 (61)100 (141)Ste
mep-1(n3703); lin-15A(n767)25 (72)100 (107)Ste
mys-1(n3681); lin-15A(n767)c0 (214)72 (192)
sli-1(n3538) lin-15A(n767)4 (138)90 (173)
sli-1(n3544) lin-15A(n767)5 (153)80 (265)cs embryonic lethality
sli-1(n3683) lin-15A(n767)5 (80)88 (148)cs embryonic lethality
trr-1(n3630); lin-15A(n767)c3 (131)85 (212)Ste, Gro
trr-1(n3637); lin-15A(n767)c1 (92)80 (200)Ste, Gro
trr-1(n3704); lin-15A(n767)c3 (96)79 (244)Ste, Gro
trr-1(n3708); lin-15A(n767)c2 (151)84 (228)Ste, Gro
trr-1(n3709); lin-15A(n767)c1 (97)77 (154)Ste, Gro
trr-1(n3712); lin-15A(n767)c6 (121)77 (192)Ste, Gro
  • The penetrance of the Muv phenotype was determined after synMuv mutant strains grew at the indicated temperature for two or more generations. For most strains for which a fully penetrant sterile phenotype was associated with the Muv phenotype, we scored the penetrance of the Muv phenotype by examining sterile progeny of heterozygous mutant parents. For trr-1 mutant strains, we scored the penetrance of the Muv phenotype by examining non-Gfp progeny of trr-1/mIn1[dpy-10(e128)mIs14]; lin-15A(n767) heterozygous parents. All strains were backcrossed to lin-15A(n767) twice prior to phenotypic characterization. In addition to the phenotypes described above, many of the strains exhibited heat-sensitive inviability as a consequence of rupture and/or general sickness. Ste, sterile; Gro, growth rate abnormal; Rup, rupture at the vulva; cs, cold sensitive; hs, heat sensitive. The characterization of some of these strains was previously described by:

  • a Ceol and Horvitz (2001),

  • b Thomas et al. (2003),

  • c Ceol and Horvitz (2004).