TABLE 2

Plasmids used in this study

NameDescription
pMW489pRS415-DBF4
pMW490pRS416-DBF4
pMW524pRS415-DBF4(NcoI)
pMW526pRS415 dbf4-ΔN65
pMW538pMW526 S84A, S92A, T95A, T105A
pCG3pRS415 dbf4-W202E
pCG8pRS415 dbf4-W202A
pCG9pRS415 dbf4-ΔN87
pCG10pRS415 dbf4-ΔN109
pCG11pRS415 dbf4-ΔN128
pCG12pRS415 dbf4-ΔN158
pCG13pRS415 dbf4-ΔN206
pCG14pRS415 dbf4-ΔN265
pCG15pRS415 dbf4-Δ136-221
pCG17pRS415 dbf4-ΔN186
pCG18pRS415 dbf4-ΔN229
pCG23pRS415 dbf4-ΔN292
pCG24pRS415 dbf4-T163A
pCG25pRS415 dbf4-ΔN172
pCG29pRS415 dbf4-ΔN221
pCG36pRS415 dbf4-Y139A
pCG37pRS415 dbf4-G159V
pCG65pRS415 dbf4-ΔN136
pCG66pRS415 dbf4-G159L, A160L
pCG76pRS415 dbf4-NLSΔN221
pCG77pRS415 dbf4-F165A
pCG78pRS415 dbf4-F165S
pCG80pRS415 dbf4-Δ71-221
pCG81pRS415 dbf4-Y127A
pCG82pRS415 dbf4-Y127S
pCG86pRS415 dbf4-NLSΔN292
pCG88pRS415 dbf4-NLSΔN265
pCG99pRS415 dbf4-Δ188-221