Plasmids used in this study
| Name | Description |
|---|---|
| pMW489 | pRS415-DBF4 |
| pMW490 | pRS416-DBF4 |
| pMW524 | pRS415-DBF4(NcoI) |
| pMW526 | pRS415 dbf4-ΔN65 |
| pMW538 | pMW526 S84A, S92A, T95A, T105A |
| pCG3 | pRS415 dbf4-W202E |
| pCG8 | pRS415 dbf4-W202A |
| pCG9 | pRS415 dbf4-ΔN87 |
| pCG10 | pRS415 dbf4-ΔN109 |
| pCG11 | pRS415 dbf4-ΔN128 |
| pCG12 | pRS415 dbf4-ΔN158 |
| pCG13 | pRS415 dbf4-ΔN206 |
| pCG14 | pRS415 dbf4-ΔN265 |
| pCG15 | pRS415 dbf4-Δ136-221 |
| pCG17 | pRS415 dbf4-ΔN186 |
| pCG18 | pRS415 dbf4-ΔN229 |
| pCG23 | pRS415 dbf4-ΔN292 |
| pCG24 | pRS415 dbf4-T163A |
| pCG25 | pRS415 dbf4-ΔN172 |
| pCG29 | pRS415 dbf4-ΔN221 |
| pCG36 | pRS415 dbf4-Y139A |
| pCG37 | pRS415 dbf4-G159V |
| pCG65 | pRS415 dbf4-ΔN136 |
| pCG66 | pRS415 dbf4-G159L, A160L |
| pCG76 | pRS415 dbf4-NLSΔN221 |
| pCG77 | pRS415 dbf4-F165A |
| pCG78 | pRS415 dbf4-F165S |
| pCG80 | pRS415 dbf4-Δ71-221 |
| pCG81 | pRS415 dbf4-Y127A |
| pCG82 | pRS415 dbf4-Y127S |
| pCG86 | pRS415 dbf4-NLSΔN292 |
| pCG88 | pRS415 dbf4-NLSΔN265 |
| pCG99 | pRS415 dbf4-Δ188-221 |