Plasmids used in this study
| Plasmid | Characteristic | Source |
|---|---|---|
| pSMA111 | PGAL10-GIN11 URA3 CEN6 | Kawahata et al. (1999) |
| pRS316-PGAL7-LacZ | PGAL7-LacZ URA3 CEN6 | This study |
| pRS314 | TRP1 CEN6 | Sikorski and Hieter (1989) |
| pRS314-BNI1 (pKY1226) | BNI1 TRP1 CEN6 | Kadota et al. (2004) |
| pRS314-bni1-116 (pKT1426) | bni1-116 TRP1 CEN6 | This study |
| pRS314-bni1(D1511G) (pKT1427) | bni1(D1511G) TRP1 CEN6 | This study |
| pRS314-bni1-FH2#1 (pKT1428) | bni1-FH2#1 TRP1 CEN6 | This study |
| pRS314-BNI1(Δ6-642) (pKT1419) | BNI1(Δ6-642) TRP1 CEN6 | This study |
| pRS314-BNI1(Δ826-986) (pKT1421) | BNI1(Δ826-986) TRP1 CEN6 | Kadota et al. (2004) |
| pRS314-BNI1(1−1750) (pKT1422) | BNI1(1−1750) TRP1 CEN6 | Kadota et al. (2004) |
| pRS314-BNI1(Δ1228-1414) (pKT1423) | BNI1(Δ1228-1414) TRP1 CEN6 | Kadota et al. (2004) |
| pRS314-BNI1(Δ1553-1646) (pKT1424) | BNI1(Δ1553-1646) TRP1 CEN6 | Kadota et al. (2004) |
| pRS316-PGAL1-HA | PGAL1-HA URA3 CEN6 | Kamei et al. (1998) |
| pRS316-PGAL1-HA-BNI1(1227-1953) (pKT1605) | PGAL1-HA-BNI1(1227-1953) URA3 CEN6 | This study |
| pBTM116-HA | DBDLexAa-HA TRP1 2 μm | Imamura et al. (1997) |
| pBTM116-HA-PFY1 | DBDLexA-HA-PFY1 TRP1 2 μm | Gift from Y. Takai |
| pBTM116-HA-BUD6(221-788) (pKT1571) | DBDLexA-HA-BUD6(221-788) TRP1 2 μm | This study |
| pBTM116-HA-BNI1(1227-1953) (pKT1572) | DBDLexA-HA- BNI1(1227-1953) TRP1 2 μm | This study |
| pBTM116-HA-BNI1(1227-1750) (pKT1573) | DBDLexA-HA-BNI1(1227-1750) TRP1 2 μm | This study |
| pACTII-HK | ADGAL4a LEU2 2 μm | Ozaki et al. (1996) |
| pACTII-HK-ACT1 (pKT1574) | ADGAL4-ACT1 LEU2 2 μm | This study |
| pACTII-HK-ACT1-301 (pKT1575) | ADGAL4-act1-301 LEU2 2 μm | This study |
| pRS313-sla2Δ33-359 (pDD367) | sla2Δ33-359 HIS3 CEN6 | Yang et al. (1999) |
| pRS313-sla2Δ33-501 (pDD364) | sla2Δ33-501 HIS3 CEN6 | Yang et al. (1999) |
| pRS313-sla2Δ33-750 (pDD362) | sla2Δ33-750 HIS3 CEN6 | Yang et al. (1999) |
| pRS313-sla2Δ360-575 (pDD371) | sla2Δ360-575 HIS3 CEN6 | Yang et al. (1999) |
| pRS316 | URA3 TRP1 | Sikorski and Hieter (1989) |
| pRS316-ACT1 (pKT1412) | ACT1 URA3 CEN6 | This study |
| pRS316-ACT1-301 (pKT1576) | act1-301 URA3 CEN6 | This study |
| pRS316-BNI1 (pKT1227) | BNI1 URA3 CEN6 | Kadota et al. (2004) |
| pRS305 | LEU2 | Sikorski and Hieter (1989) |
| pRS305-PGAL1-HA-BNI1(1227-1953) (pKT1606) | PGAL1-HA-BNI1(1227-1953) LEU2 | This study |
↵a DBDLexA and ADGAL4 are the DNA-binding domain of LexA and the transcriptional activating domain of Gal4p, respectively.