TABLE 3

Chromosome III loss and recombination in hda3 mutants

Chromosome loss frequencya (×10−6)Recombination frequencyb (×10−6)
Genotype30°14°30°14°
HDA3/HDA31.22.61.53.7
hda3Δ/hda3Δ105652725
  • Frequencies of mitotic recombination and loss of chromosome III in diploids were determined as described in materials and methods. Frequencies were derived by fluctuation analysis from 5 to 10 individual cultures of each strain. Values shown are derived from one set of data. The experiment was repeated at least once for each condition with similar results. The strains used were IPY360 and IPY361.

  • a The frequency of chromosome loss was determined by scoring for the loss of the URA3 marker (growth on 5-FOA medium) at the his4 locus, located on the right arm of chromosome III in conjunction with the loss of the respective MAT locus (mater) on the left arm of chromosome III.

  • b The frequency of mitotic recombination was determined by scoring for the loss of the URA3 marker (growth on 5-FOA medium) at the his4 locus, located on the right arm of chromosome III, and the maintenance of both MATa/MATα loci (nonmater) on the left arm on chromosome III.