TABLE 2

Receptor guanylyl cyclases in C. elegans

Other namesaMap positionbExpression pattern in adult animals
GenePrevious reportsThis report
daf-11B0240.3V +3.27ASIL/R, ASJL/R, ASKL/R, AWBL/R, AWCL/Rc
gcy-1AH6.1II +1.49No signaldASER, ASIL/R, PVT, URXL/R, AIYL/R, intestine
gcy-2R134.2II +1.48AWAL/R, ASIL/R, RIAL/R, PVT
gcy-3R134.1II +1.48ASER, ASIL/R, PVT
gcy-4ZK970.5II +2.31No signaldASER biasedh
gcy-5ZK970.6II +2.32ASERd
gcy-6B0024.6V +2.46ASELd,g
gcy-7F52E1.4V +1.37ASELd,gAlso expressed in excretory canal cell (only in adults)
gcy-8C49H3.1IV +3.50AFDL/Rd
gcy-9ZK455.2X +3.0No signaldWeak, occasional, and variable expression in non-neuronal tissues
gcy-11C30G4.3X +24.07Pharyngeal muscle
gcy-12F08B1.2II −1.80PHAL/Rd
gcy-13F23H12.6V +4.02No signaldRIML/R
gcy-14ZC412.2V +6.93No signaldASEL biased,h AWCL/R (faint), PVT
gcy-15ZC239.7II −8.08ASGL/R (faint)
gcy-17W03F11.2, gcy-24I −10.91PHAL/R
gcy-18ZK896.8, gcy-26IV +6.48AFDL/R, AIML/Ri
gcy-19C17F4.6II −7.80IL2 (strong), ASEL/R (faint),j additional faint sensory neurons (three pairs)
gcy-20F21H7.9, gcy-16V +9.87ASEL, AWCL/R (faint) excretory gland and canal cells
gcy-21F22E5.3II -12.28ASGL/R, ADLL/R (faint)
gcy-22T03D8.5V +25.25ASERf
gcy-23T26C12.4IV -5.01AFDL/Ri
gcy-25Y105C5B.2IV +14.17AQR, PQR, URXL/R
gcy-27C06A12.4IV +16.74ASKL/R, ASIL/R, ASJL/R
gcy-28T01A4.1I −1.17Many head neurons, ventral cord and tail neurons, body-wall muscle, hypodermis, somatic gonad, intestinek
gcy-29C04H5.3II +23.04ASEL/R, AWCL/R, AVKL/R, AFDL/R, few variable other neurons (weak)
odr-1R01E6.1, gcy-10X +12.7ASIL/R, ASJL/R, ASKL/R, AWBL/R, AWCL/Rd,e
  • Previously characterized and newly determined receptor gcy expression patterns are summarized. —, indicates that expression was not analyzed.

  • a See materials and methods for comments on gene nomenclature.

  • b From http://www.wormbase.org.

  • c Birnby et al. (2000).

  • d Yu et al. (1997). We suppose that we detected clear expression in several cases where no signal was observed by Yu et al. (1997) since we (1) used a gfp variant that is much brighter than the old gfp version used by Yu et al. (1997) and (2) since our reporter constructs may encompass more cis-regulatory sequences than those of Yu et al. (1997).

  • e L'Etoile and Bargmann (2000).

  • f Johnston et al, (2005). Additional weak expression that fades in adults is observed in two additional, unidentified head neurons.

  • g As described in Johnston et al. (2005) gcy-6 and gcy-7 are embryonically expressed in both ASEL and ASER and only become restricted to ASEL postembryonically. A similar scenario may apply for other ASEL-expressed gcy genes, but has not been explicitly examined.

  • h “ASER biased” incorporates two categories of expression patterns in a given transgenic line: expression only in ASER in some animals and stronger expression in ASER than in ASEL in other animals. The opposite holds for ASEL-biased expression. Such weak and occasional expression in the other cell could be caused by array-overexpression artifacts and we therefore do not want to emphasize that biased expression is fundamentally different from exclusive expression.

  • i After the initial submission of this article for publication, Inada et al. (2006) also described the expression pattern of gcy-18 and gcy-23 in the AFD neurons.

  • j Expression in ASEL/R is very dim and not completely penetrant and potential biases to ASEL or ASER are therefore difficult to determine.

  • k Expression in all tissue types is very mosaic. Expression in the ASE and AWC neuron classes could not be observed, but as with any other reporter construct described in this table it is possible that additional regulatory elements not contained within the respective constructs may yield expression in these neurons.