TABLE 2

ZFN-induced NHEJ mutation frequencies

Female germlineMale germline
LocushsTParentsYieldersMutantsParentsYieldersMutants
y35°125002281324
bw35°2280024566
ry36.5°573189502245
38°80715047852534
  • For each experiment, the heat-shock temperature (hsT) is indicated. The number of female and male parents subjected to the heat shock is given, followed by the number that yielded at least one mutant offspring (Yielders), and the total number of mutants recovered. The data for y are from Bibikova et al. (2002).