TABLE 1

Zinc-finger sequences

ZFNTarget (5′ → 3′)Finger 1Finger 2Finger 3
yAGTG GAT GAGRSDNLVRTSGNLVRRSDALVRa
yBGCG GTA GGCDPGHLVRQSSSLVRRSDERKRb
bwAGAT GAT GGGRSDKLVRTSGNLVRTSGNLVR
bwBGAG GTG GGCDPGHLVRRSDELVRRSDNLVR
ryAGGC GTG GGAQSGHLQRcRSDALTRcDPGHLVR
ryBGTA GTA GCTQSSDLTRcQSSSLVRQSSSLVR
  • The specificity-determining residues [from position −1 to +6 in the standard notation (Segal 2002)] for each set of zinc fingers are given in the one-letter code. In reading the table, it is important to remember that the first finger binds the 3′-most DNA triplet. For example, the GAG triplet in the yA target is recognized by Finger 1 (RSDNLVR). Finger sequences without footnotes were derived and characterized in the Barbas lab (Segal et al. 1999).

  • a This finger for GTG was obtained from the Barbas lab, but is not listed in their collection of derived fingers (Segal et al. 1999; Segal 2002).

  • b This finger for GCG is a natural sequence from the transcription factor Zif268 (Pavletich and Pabo 1991).

  • c These fingers are from the Sangamo collection (Liu et al. 2002).