TABLE 2

Oligonucleotides used in this study

PrimerSequencePosition/purpose
OKH60gac tct ata cca ccg tac gcc gat atForward primer for argB+
OKH61cac cgg gtg cga ttt gcc cca ttt ccReverse primer for argB+
OYG7ggt gct cat ccc gtg tca aac a5′-Flanking forward primer of sfgA
OYG8gct aat tgg tat ttt gcc tct a5′-Flanking nested forward primer of sfgA
OYG9agt caa atg agg cct cta aac tgg tc a5′-Flanking reverse primer of sfgA with argB tail (underlined)
aga gct gta agg gag aga cat g
OYG10agc caa ggt aga tcc agg cct aac ac a3′-Flanking forward primer of sfgA with argB tail (underlined)
gac cat aca cct cgc act ttc t
OYG11cat gga ctg aat aat gtg gct c3′-Flanking reverse primer of sfgA
OYG12cta tct ata att aag cca agc a3′-Flanking nested reverse primer of sfgA
OYG13acc tcg ccc ctc cat gtc tct cSequencing of sfgA, RT–PCR
OYG21cta tat aga cgc cgt aac caa cConfirmation of ΔsfgA, RT–PCR
OYG22atc tca gat cat ctc atc aag aConfirmation of ΔsfgA
OJA107aca atc aat gtt caa tgt ac5′ end of alcA(p)
OJA106ttt gag gcg agg tga tag gat tgg a3′ end of alcA(p)
OJA108cg gga tcc agt ggt tcg gta atcalcA(p) 5′ nested with BamHI tail (boldface italic)
OYG28tcc aat cct atc acc tcg cct caa a3′ end of alcA(p) (underlined) + 5′ forward of sfgA ORF
atg gaa gct cgt acg atg gt
OYG29cg ggatc cta tct ata att aag cca agc a3′ nested of sfgA with BamHI (boldface italic)
OYG30atg tgc tac cag tat gct5′ forward of flbD
OYG31tcg tag cgt gcc tag gta3′ reverse of flbD
OYG38acttgatcatcgctccaa5′ forward of flbC
OYG39gaaagccgaatcgtacac3′ reverse of flbC
OJA133gag att cga gcc tgt gc5′ forward of flbA
OJA134ctg tca tga acg ttg tg3′ reverse of flbA
OJA142ctg gca ggt gaa caa gtc5′ forward of brlA
OJA143aga agt taa cac cgt aga3′ reverse of brlA
OJA152aac tct cga gct gac atg5′ forward of yA
OJA153ctt gga cat tat gta ggt3′ reverse of yA