TABLE 3

zds1 expression reverses the sporulation deficiency of the ras1 strain but has no effect on byr2, byr1, and spk1 sporulation

Sporulation rate (%)
HostPlasmid+Thiamine−Thiamine
SPRN1DA (ras1/ras1)pREP10.10.3
pREP1-zds10.711.2
pREP410.10.7
pREP41-zds10.22.9
SPSUD (byr2/byr2)pREP100
pREP1-zds100
SPBUD (byr1/byr1)pREP100
pREP1-zds100
SPKUD (spk1/spk1)pREP100
pREP1-zds100
  • The strains were streaked onto SPA plate and grown at 30° for 2 days. About 1000 cells were counted under the microscope.