TABLE 2

Oligonucleotide primers used in this study

PrimerSequence
SAMH601F5′-ATGTCGAC (SalI) AGAATTC (EcoRI) ATGTCTAGTTCTTCAGTT-3′
SAMH601R5′-ATGTCGAC (SalI) GGATCC (BamHI) TTTAGCATTCTTTGAAAC-3′
zds1-GFP55′-ATGAATTC (EcoRI) GCGGCCGC (NotI) ATGTCTAGTTCTTCAGTT-3′
zds1-GFP35′-ATGAATTC (EcoRI) GCGGCCGC (NotI) TTTTAGCATTCTTTGAAAC-3′
PH1–1795′-AATTTAAAGCGGCCGC (NotI) CGGTAGATGCATCTTGTTC-3′
PH1–2265′-TTTTAAAAGCGGCCGC (NotI)CTAGGGAACGAATGTGCTC-3′
PH1–5675′-ATATGCATGCGGCCGC (NotI) CATAACCCTCAGTTTTATT-3′
PH1–6695′-ATAAATTTGCGGCCGC (NotI)CACCTTTGTTAGCAGTGGA-3′
PH1–8175′-GGCATATTGCGGCCGC (NotI)CACTCCAGTAATAATCCTT-3′
PH194–9385′-AATCGAAAGCGGCCGC (NotI) ATGACTGGTTGGAAATCGTTT-3′
PH239–9385′-TTTTAAAAGCGGCCGC (NotI) ATGGCTGACGATGCTGTAATT-3′
PH573–9385′-ATGCAGCTGCGGCCGC (NotI) ATGCGTGAGGAAAAGAATTAT-3′
PH682–9385′-TAAATTTTGCGGCCGC (NotI)ATGAAGAAAACCAAGAGGTCC-3′
  • Restriction enzyme sites are underlined.