TABLE 2

Estimates of the distribution of mutational effects for variance-weighted data from D. miranda and D. pseudoobscura

cn (%)αlocSpeciesNes (am)Nes (hm)Nes (5%)Nes (95%)CVcne (%)Embedded Image (%)Embedded Image (%)
00.299 (0.0782/0.741)0.000266 (1.14 × 10−5/17,000)mir255 (11.6/95,700)8.35 (3.49/17.9)1.31 (0.843/3.01)1,100 (36.9/558,000)1.67 (1.08/2.15)12.7 (7.91/17.0)−42.8 (−125/14.7)0.902 (−83.5/60.3)
263 (12.4/97,500)13.3 (4.82/31.7)2.22 (1.40/5.06)1,100 (37.8/560,000)1.64 (1.02/2.11)15.5 (11.0/20.2)
pso1,370 (55.3/521,000)14.4 (8.69/25.0)2.50 (1.55/5.47)6,220 (188/3,110,000)1.74 (1.14/2.22)7.57 (2.35/13.4)15.7 (−72.1/73.7)
1,400 (56.2/534,000)23.0 (11.8/43.8)3.72 (2.39/8.47)6,240 (188/3,200,000)1.72 (1.12/2.20)9.19 (3.65/14.7)
2.50.448 (0.0996/1.32)7.67 × 10−5 (7.12 × 10−6/89.5)mir70.6 (6.96/76,500)6.86 (3.19/17.2)1.21 (0.877/2.96)274 (18.4/463,000)1.39 (0.832/2.08)11.4 (5.73/16.6)−31.1 (−117/27.9)7.93 (−78.7/57.7)
73.1 (7.32/78,800)10.1 (3.99/29.9)1.93 (1.37/4.80)275 (18.8/476,000)1.36 (0.789/2.05)14.4 (9.04/19.3)
pso382 (35.4/392,000)14.7 (9.48/28.4)2.73 (1.77/7.31)1,550 (97.2/2,450,000)1.45 (0.865/2.13)6.58 (2.87/13.4)16.4 (−70.0/59.1)
388 (35.4/401,000)21.5 (12.2/45.5)3.96 (2.59/9.77)1,550 (97.2/2,490,000)1.43 (0.857/2.12)7.95 (3.30/14.6)
50.831 (0.228/1.75)2.33 × 10−5 (6.84 × 10−6/0.0179)mir20.4 (6.53/11,400)5.42 (3.39/18.9)1.21 (0.931/3.53)63.6 (15.8/55,700)1.05 (0.737/1.95)9.27 (6.40/16.1)−11.2 (−113/22.6)15.2 (−78.3/38.3)
21.1 (6.72/11,600)7.07 (4.08/31.5)1.76 (1.42/5.40)63.7 (16.2/55,800)1.02 (0.702/1.93)12.2 (8.41/19.0)
pso112 (31.7/80,400)17.4 (10.5/35.2)4.28 (2.13/10.9)362 (80.1/413,000)1.09 (0.753/1.98)6.01 (5.08/12.1)14.4 (−73.1/35.6)
113 (32.0/81,200)21.5 (12.5/53.0)4.73 (2.93/13.1)362 (80.2/415,000)1.08 (0.746/1.97)6.72 (5.22/13.7)
  • The Nes values reported here describe the distribution of deleterious mutational effects for all effectively nonneutral and nonlethal sites, where (am) denotes the arithmetic mean and (hm) the harmonic mean; (5%) and (95%) represent the lower and upper fifth percentiles of this truncated distribution; mir and pso refer to D. miranda and D. pseudoobscura, respectively; cn is the fraction of completely neutral mutations assumed in the calculations; cne is the fraction of effectively neutral mutations; CV denotes the coefficient of variation of Nes values of the truncated distribution; Embedded Image, Embedded Image, and Embedded Image are the proportions of adaptive amino acid substitutions, assuming either the current population size of D. miranda or D. pseudoobscura or their mean as the ancestral Ne, respectively. For a given species and value of cn, the upper and lower values for all estimates of Nes, cne, and CV correspond to assumed borders to neutrality of Nes = 0.5 and 1, respectively. Values in brackets indicate the lower and upper approximate fifth percentiles from bootstrapping across genes, after omitting all values that did not fit the data (see text). All estimates assume a mutational bias of κ = 2, a mutation rate of μ = 1.5 × 10−9, and a gamma distribution of mutational effects with shape parameter α and location parameter β, where β is computed from loc, the arithmetic mean of the untruncated distribution of selection coefficients given in the table (see text).