TABLE 5

Synthetic defect in fbp1-lacZ derepression by cgs2-s1 and gpa2R176H

β-Galactosidase activity
StrainGenotypeRepressedDerepressed
FWP72gpa2+ cgs2+3 ± 01369 ± 142
CHP733gpa2+ cgs2-s14 ± 0263 ± 12
RWP1gpa2R176H cgs2+2 ± 0483 ± 5
CHP732gpa2R176H cgs2-s13 ± 012 ± 3
CHP384gpa2+ cgs2-23 ± 024 ± 2
  • Cells were grown in PM complete medium and β-galactosidase activity was determined from three independent cultures as described in materials and methods. The average ±SE represents specific activity per milligram of soluble protein.