TABLE 3

Suppression of git3Δ by cgs2-s1 and cgs2-2 in haploid and diploid strains

β-Galactosidase activity
StrainGenotypeRepressedDerepressed
FWP72git3+ cgs2+3 ± 01369 ± 142
RWP9git3Δ cgs2+925 ± 101282 ± 10
LWP167git3Δ cgs2-s127 ± 10527 ± 56
LWP161git3Δ cgs2-24 ± 052 ± 20
LWP127 × LWP110git3+/git3+ cgs2+/cgs2+6 ± 11754 ± 32
LWP156 × CHP934git3Δ/git3Δ cgs2+/cgs2+513 ± 1301573 ± 32
LWP156 × CHP769git3Δ/git3Δ cgs2+/cgs2-s168 ± 15970 ± 234
LWP156 × LWP159git3Δ/git3Δ cgs2+/cgs2-24 ± 143 ± 22
  • β-Galactosidase activity was determined from three independent cultures as described in materials and methods. The average ±SE represents specific activity per milligram of soluble protein.