Synthetic effects of swi6 and mediator mutants grown at different temperatures

25°30°37°Suppression at HO-lacZ
Tail domain
SWI6 sin4++
swi6 sin4+/−+/−+
SWI6 pgd1++++++
swi6 pgd1(+)+(+)+
SWI6 gal11++++++
swi6 gal11++++++
Middle domain
SWI6 nut1++++++
swi6 nut1+++++++
SWI6 med1+++++
swi6 med1++(+)+
SWI6 med9++++++
swi6 med9(+)(+)(+)+
SWI6 srb7++++++
swi6 srb7++++(+)+
Kinase complex
SWI6 srb8++++++
swi6 srb8++++++
SWI6 srb9++++++
swi6 srb9++++++
SWI6 srb10++++++
swi6 srb10+++++++
SWI6 srb11++++++
swi6 srb11++++++
Head domain
SWI6 srb2++++++
swi6 srb2+/−+/−+/−ND
SWI6 srb5+++
swi6 srb5+/−+/−+/−ND
SWI6 rox3++++++
swi6 rox3+++++++
  • The growth phenotypes of mediator subunit mutants in a swi6 background grown on YEPD plates were scored from best to worst as follows: ++, +, (+), +/−, −. ND, not determined. The suppression phenotype of each double mutant is indicated as “+” if the mediator defect restores HO-lacZ expression on the basis of the X-Gal filter assay described in materials and methods.