TABLE 1

Brc1 suppression of smc6-74: summary of suppression of smc6-74 by Brc1 overexpression

Allele testedMMSUV
chk1Δ++++++++++
cds1Δ++++++++++
rad13-A++++++++++
rqh1Δ++++++++++
rhp18Δ
slx1Δ+/−+/−
mus81Δ++++++
ubc13Δ++++++++++
TLS 3Δ+++++
dinBΔ (pol κ)++++++++
rad3Δ++++++
rad9Δ++++++
rad17Δ+ND
rhp57Δ
swi5-39+++++++
rhp51ΔEpistaticEpistatic
rhp54ΔEpistaticEpistatic
rad2-44EpistaticEpistatic
rad32Δ++++++++++
  • MMS sensitivity was determined over a range of concentrations appropriate for parental strains. UV-C sensitivity was determined from 0 to 250 J/m2. Degree of rescue was estimated from survival curves. +++++, rescue to sensitivity of allele tested (>95% suppression); —, same as vector-only control (0% suppression); +/−, marginal (<5%) suppression; and +, 5–20%, ++, 20–40%, +++, 40–80%, and ++++, 80–95% suppression. ND, not determined.