TABLE 3

Molecular structure of Lamin C excision alleles

Mutant nameCoding regionNo. of insertionsInsertion size (kb)GFPawhite+/−b
G00158 (parent stock)Intact16++
LamCEX3Intact11.5
LamCEX5Intact11.8
LamCEX16Intact11.5
LamCEX187357-bp deletion in first exon2ND+
LamCEX265Intact11.4
LamCEX296560-bp deletion in first exon0NA
LamCEX364Intact1ND+
  • ND, not done; size exceeded standard PCR. NA, not applicable.

  • a Determined by PCR.

  • b Determined by eye color phenotype indicating presence of white reporter (+, red; −, white).