TABLE 4

Quantification of FM4-64 uptake: sporulating cells

Time (min) after
 removal of FM4-64Plasma
 membranePunctateVacuolesProspore
 membranesFaint cytoplasm
 or no signal
END3: % of cells labeled with FM4-64
 030.646.621.4 0.6 0.8
1017.451.026.2 3.6 1.8
20 6.663.224.2 4.8 1.2
30 7.231.945.312.2 3.4
6010.0 2.266.6a18.6 2.6
end3Δ: % of cells labeled with FM4-64
 090.8 1.2 0.0 3.4 4.6
1066.816.8 0.2 2.813.4
2058.825.1 9.8 2.2 4.1
3029.931.321.011.4 6.4
6016.4 5.856.0a14.8 7.0
  • a After 60 min 85% of wild-type and 51% of end3Δ cells displayed strong vacuolar staining.

  • Five hundred cells were counted for sporulating wild-type and end3Δ strains.