TABLE 3

Quantification of FM4-64 uptake: vegetative cells

Vacuoles
Time (min)
 after removal
 of FM4-64PunctateStrongWeakFaint cytoplasm
 or no signal
END3: % of cells labeled with FM4-64
 098.7 0.0 0.0 1.3
1085.3 0.7 6.7 7.3
2022.160.311.4 6.2
3010.065.323.0 1.7
60 0.062.336.0 1.7
end3Δ: % of cells labeled with FM4-64
 0 0.0 0.0 1.099.0
1017.7 0.7 3.777.9
20 2.3 2.025.070.7
30 0.7 6.053.340.0
60 0.735.356.7 7.3
  • Five hundred cells were counted for vegetative wild-type and end3Δ strains.