TABLE 2

Sporulation efficiency

No. of spores/cell
StrainRelevant
 genotypeTemperature43a210
AN120END3/END324°68.7 (9.3)20.3 (4.2)5.4 (2.7)0.3 (0.2) 5.2 (3.2)
30o65.6 (2.7)19.2 (3.9)1.2 (0.5)0.2 (0.2)13.7 (4.9)
33°70.6 (3.7)26.6 (3.8)0.0 (0.0)0.2 (0.4) 2.7 (0.6)
Y4733end3Δ/end3Δ24°25.8 (2.8)28.1 (2.4)16.8 (2.9)9.8 (3.3)19.6 (8.3)
30°3.5 (0.5)11.8 (3.5)11.5 (4.1)17.3 (3.1)55.9 (10.0)
33°3.3 (1.2)8.0 (1.8)14.3 (2.3)26.0 (3.5)48.3 (6.1)
  • a Values may be overrepresented since tetrads and three-spored asci are indistinguishable in wild-type cells, while three-spored asci in end3Δ mutant cells are clearly distinguished from tetrads. Standard deviation for each value is shown in parentheses. n = 300.

  • Frequencies of spore formation are shown as percentages.