TABLE 1

Summary of deleterious mutations

MutationGeneAminoPlaque
 sizeRelative
 fitnessnnbackncomp
G232C CE34Q0.8030.0108 16 0 14
T489C DF34L2.4460.7946 1616  0
T495C DF36L2.2700.5609 1616  0
G1132C FM43L2.8180.1485 16 3 13
C1137T FA45V3.0200.3151 16 3 13
C1280T FP93S3.6590.4081 16 3  0
G1423C FM140I2.1860.3257 16 5 11
A1624T FE207D1.0920.0195 16 3a 12
T1631C FY210H4.2790.4509  8 2  2
A1757T FT252S1.6710.0295  8 1  7
C2235T FT411I3.1610.2214  8 1  5
C2480T GA29V1.8810.1205 16 0 12
G2746C GG118R0.2440.0004  8 0  8
G2979Ab HA17T2.2220.4283  8 5  3
A3054C HN42H1.9200.4901 16 0 16
T3288C HY120H2.3170.1405  8 4  4
G3326T HE132D1.1240.0101 16 2 14
G3524C HM198I3.0210.5071 16 0 16
A3582G HN218D3.8730.5385 16 0  0
C4552A AA191D1.6320.1589  8 8  0
G4970T AE330D3.7430.4895 16 0  0
A5031Gb AK351E1.0800.0197 16 8  8
C5337A A, BL453I, A88D1.3180.0230 16 2 14
300aI4.5641.0
Totals:
31282172
  • a Includes two independent same-site reversions to nonancestral nucleotide (T1624G).

  • b Does not introduce DpnII restriction site.

  • Each mutation is identified by the initial nucleotide, genome position (numbered from the PstI restriction site in accordance with Sanger et al. 1978), and final nucleotide. “Amino” refers to amino acid substitutions (initial amino acid, position, and final amino acid). “Plaque size” (i.e., area) is reported in units of millimeters squared. “Relative fitness” is estimated from the average phage yield per plaque of the deleterious mutation, standardized by the yield of ancestral 300aI. The total number of replicate populations founded from each deleterious mutation is listed under n. The numbers of populations acquiring back or compensatory mutations are listed under nback and ncomp, respectively.