TABLE 1

The effect of host MHC, parasite clone, and host gender on the time course of disease symptoms

Disease symptom
ParasitemiaBlood cell countsBody weight change
Fd.f.PFd.f.PFd.f.P
Between subjects
Host MHC  9.6 2, 940.0002  5.2 2, 940.007 1.2 2, 940.29
Plasmodium clone  5.9 1, 940.017  0.1 1, 940.79 3.4 1, 940.07
Host gender  0.02 1, 940.89 27.8 1, 94<0.0001 0.1 1, 940.77
MHC × clone  0.8 2, 940.43  0.5 2, 940.61 0.3 2, 940.75
MHC × gender  0.9 2, 940.39  1.2 2, 940.32 0.2 2, 940.82
Clone × gender 10.1 1, 940.002  9.5 1, 940.003 0.4 1, 940.52
MHC × clone × gender  0.7 2, 940.49  1.2 2, 940.31 1.2 2, 940.31
Within subjects (repeated measurements on individual mice)
Time296.7 5, 90<0.0001166.110, 85<0.000133.118, 77<0.0001
Time × MHC  2.410, 1800.01  1.220, 1700.26 1.036, 1540.46
Time × clone  6.7 5, 90<0.0001  2.910, 850.003 1.718, 770.05
Time × gender  1.5 5, 900.20  1.910, 850.06 6.218, 77<0.0001
Time × MHC × clone  0.410, 1800.92  0.620, 1700.94 1.236, 1540.27
Time × MHC × gender  0.710, 1800.72  1.020, 1700.46 0.836, 1540.78
Time × clone × gender  1.1 5, 900.38  1.710, 850.10 1.918, 770.03
Time × MHC × clone × gender  1.410, 1800.18  0.720, 1700.86 0.836, 1540.72
  • The experiment was designed for a fully factorial repeated-measures analysis of variance (ANOVA), incorporating the fixed-effect factors “host MHC,” “host gender,” and “Plasmodium clone” with repeated measures of the following dependent variables: parasitemia (6 measurements from day 4 to day 14), blood cell counts (11 measurements from day 1 to day 22), and body weight (19 measurements from day 4 to day 22), given as differences from the weight at day 0. For within-subject analyses, we used the multivariate F-tests or Wilk's λ (when a factor had more than two levels as in “MHC”).