TABLE 2

Mos1 insertion alleles

ScreenTransposition
 frequencyNo. of F1'sMutantsInsertion site
118/31 (0.58) 2,300lev-1(kr6)Exon 3
unc-63(kr13)Splice donor
216/23 (0.70) 5,700lev-1(kr20)Exon 3
unc-63(kr19)Exon 7
311/30 (0.37) 5,900lev-1(kr25)Promoter
lev-10(kr26)Exon 7
Total45/84 (0.53)13,900Six mutants (frequency = 4.32 × 10−4)
EMS 1,710Eight mutants (frequency = 4.68 × 10−3)
  • Adult P0 transgenic animals containing both enzyme and substrate arrays were heat-shocked to activate transposition. Efficiency of Mos1 transposition was determined in a sample of the F1 progeny. Young-adult F2 worms were screened for resistance to 1 mm levamisole 3–5 hr after transfer to drug-containing plates. After 2–5 outcrosses, Mos1 insertions were localized by inverse PCR as previously described (Bessereau et al. 2001). Gene identities were confirmed by complementation tests. In a parallel screen, mutagenesis was performed using 47 mm EMS (Brenner 1974).