TABLE 1

Transposition frequency within different classes of F1 animals

Substrate array (+)Substrate array (−)Total
Enzyme array (+) 70/137 (0.51)44/229 (0.19)114/366 (0.31)
Enzyme array (−) 59/152 (0.39)26/141 (0.18) 85/293 (0.29)
Total129/286 (0.45)70/370 (0.19)199/659 (0.30)
  • Adult P0 worms containing both substrate and enzyme arrays were heat-shocked and F1 animals were scored for the presence of each array on the basis of expression of GFP markers. Mos1 insertions were detected by PCR. Results are presented as Mos1 insertion positive F1 animals/total number of F1 animals scored (transposition frequency). Values are the sum of three independent experiments. The transposition frequency within each class was similar in all three experiments. Significance was determined by using chi-square: substrate array (+) vs. substrate array (−), χ2 = 35.3, P < 0.001 with 1 d.f.; enzyme array (+) vs. enzyme array (−), χ2 = 0.19, P > 0.50.