TABLE 2

S. cerevisiae-E. coli shuttle plasmids

Plasmid namePlasmid
 typeYeast
 markerPromoter with
 expression cassetteSource/reference
pRS316 CEN URA3NoneSikorski and Hieter (1989)
pRS316GAL CEN URA3PGALNewnam et al. (1999) and
   references therein
pRS313 CEN HIS3NoneSikorski and Hieter (1989)
pLA1 CEN HIS3PGALNewnam et al. (1999) and
   references therein
pYCL1 CEN LEU2NoneChernoff et al. (2000)
YEp13 2μ LEU2NoneBroach et al. (1979)
pRS424 2μ TRP1NoneSikorski and Hieter (1989)
CEN-GAL-SUP35 CEN URA3PGAL-SUP35Derkatch et al. (1996)
pRS316GAL-SUP35N CEN URA3PGAL-SUP35NWegrzyn et al. (2001) and
   references therein
pLA1-SUP35 CEN HIS3PGAL-SUP35ΔSWegrzyn et al. (2001) and
   references therein
pLA1-SUP35N CEN HIS3PGAL-SUP35NWegrzyn et al. (2001) and
   references therein
pmCUP1-SUP35-HA CEN URA3PCUP1-SUP35-HAThis study
pYS104 CEN URA3PHSP104-HSP104Chernoff et al. (1999) and
   references therein
pYS-GAL104 CEN URA3PGAL-HSP104Chernoff et al. (1999) and
   references therein
pH 28 CEN HIS3PGAL-HSP104Chernoff et al. (1999) and
   references therein
pC211 CEN HIS3PSSA2-SSA1Schwimmer and Masison (2002)
pLH101 2μ LEU2PSSA1-SSA1Newnam et al. (1999) and
   references therein
pGAL-SSA1 CEN URA3PGAL-SSA1Newnam et al. (1999) and
   references therein
pRS316-K-SSA1 CEN URA3PSSA1-SSA1James et al. (1997)
pRS316-K-SSB1 CEN URA3PSSB1-SSB1James et al. (1997)
pRS316-K-A/B series CEN URA3PSSA1(or PSSB1)-SSA1/SSB1James et al. (1997)
pTEF-SSA1 CEN URA3PTEF1-SSA1James et al. (1997)
pTEF-SSB1 CEN URA3PTEF1-SSB1James et al. (1997)
pTEF-A/B series CEN URA3PTEF1-SSA1/SSB1James et al. (1997)
pRS424-SSB1 2μ TRP1PSSB1-SSB1This study
pN2 CEN HIS3PSSA2-SSA2Schwimmer and Masison (2002)
pRS316GAL-SSA3 CEN URA3PGAL-SSA3This study
pYCL1-GAL-SSA3 CEN LEU2PGAL-SSA3This study
pYCL1-GAL-SSA4 CEN LEU2PGAL-SSA4This study
pTEF-SSA4 CEN URA3PTEF1-SSA4This study
pTEF-SSA3 CEN URA3PTEF1-SSA3This study
  • See materials and methods for comments.