TABLE 1

Product formation and cell survival following HO induction in wild-type, msh2, msh6, and sgs1 A-A and F-A strains

Southern blot product formationCell survival
Relevant
 genotypeA-AF-AA-A/F-A
 ratioF-AA-AA-A/F-A
 ratio
wild type1.05 ± 0.240.30 ± 0.053.50.61 ± 0.120.19 ± 0.033.4
msh2ΔNDND0.04 ± 0.010.04 ± 0.031.0
msh2Δ10.11 ± 0.020.04 ± 0.012.90.11 ± 0.050.04 ± 0.012.8
msh2-K564E0.89 ± 0.090.57 ± 0.071.60.63 ± 0.090.31 ± 0.052.0
msh2-R730W0.81 ± 0.120.62 ± 0.061.30.60 ± 0.120.42 ± 0.091.4
msh2-S656P0.47 ± 0.080.35 ± 0.051.30.39 ± 0.020.23 ± 0.071.7
msh6Δ1.12 ± 0.260.86 ± 0.071.30.87 ± 0.020.61 ± 0.061.4
msh6-F337A1.06 ± 0.200.74 ± 0.141.40.77 ± 0.060.57 ± 0.051.4
msh6-G987D1.05 ± 0.140.86 ± 0.151.20.65 ± 0.130.64 ± 0.041.0
sgs1Δ0.91 ± 0.120.73 ± 0.041.20.79 ± 0.160.75 ± 0.181.1
sgs1-hd0.87 ± 0.100.88 ± 0.111.00.89 ± 0.130.68 ± 0.041.3
sgs1ΔC7951.21 ± 0.180.91 ± 0.111.30.90 ± 0.080.69 ± 0.131.3
sgs1ΔN6441.15 ± 0.090.90 ± 0.171.31.00 ± 0.020.75 ± 0.201.4
  • Southern blot product formation (Figures 15) and cell viability data are presented as the average of three to seven experiments ±1 SD. See materials and methods for experimental details. ND, not determined.