TABLE 4

Tetrad analysis of two-point crosses

Tetrad typesSpore 1Spore 2Spore 3Spore 4No.c
Cross X1119a: h+ ura4A-13 × h ura4A-10
A+ −+ −− +− +239
+ −+ −− +− +
B+ −+ −+ +− + 10
+ −+ −+ +− +
C+ −− −− +− +  4
+ −− −− +− +
D+ −+ −+ −− + 25
+ −+ −+ −− +
E+ −− +− +− + 10
+ −− +− +− +
F+ −+ −+ −− +  1
+ −+ −− +− +
G+ −+ −− +− +  1
+ −− +− +− +
Total:290
Cross MX1124b: h+ ura4A-13 pms1 swi10 × h ura4A-10 pms1 swi10
A+ −+ −− +− +32, 8, 13
+ −+ −− +− +
B+ −+ −+ +− + 1, 1, 0
+ −+ −+ +− +
D+ −+ −+ −− + 1, 0, 0
+ −+ −+ −− +
F+ −+ −+ −− + 3, 0, 2
+ −+ −− +− +
G+ −+ −− +− + 2, 1, 2
+ −− +− +− +
H+ −+ −+ +− + 3, 0, 0
+ −+ −− +− +
I+ −+ −− +− + 0, 2, 3
+ −+ +− +− +
K+ −+ −− +− + 0, 0, 2
+ −− −− +− +
L+ −+ −+ −− + 1, 0, 0
+ −+ +− +− +
M+ −+ −+ −− + 0, 0, 1
+ −+ +− −− +
Total:43, 12, 23
  • a For each spore of the tetrads the genotype is given for both DNA strands (+, wild type; −, mutant), first for the 5′ site (13), followed by the 3′ site (10).

  • b Octad analysis was performed on this cross. Thus, the + and − symbols refer to homoduplex DNA derived from the first mitotic division of spores.

  • c Only complete tetrads were considered for cross X1119. For cross MX1124, the numbers of complete octads for a specific type are given first, followed by the numbers of octads with one or two missing colonies (see text).