Random spore analysis of two-point crosses
Type III cross (low)c | Type IV cross (high)c | |||||
---|---|---|---|---|---|---|
Mutationsa | Distanceb (bp) | ppmd | Densitye | ppmd | Densitye | Quotientf |
13 × 1 | 72 | 620 | 8.6 | 880 | 12.3 | 0.70 |
13 × 5 | 147 | 960 | 6.5 | 2,200 | 14.8 | 0.44 |
13 × 14 | 261 | 2100 | 7.9 | 4,600 | 17.5 | 0.45 |
13 × 9 | 371 | 6900 | 18.5 | 7,900 | 21.2 | 0.87 |
13 × 2 | 481 | 6800 | 14.1 | 9,500 | 19.8 | 0.71 |
13 × 10 | 605 | 8600 | 14.2 | 12,000 | 20.2 | 0.71 |
1 × 5 | 75 | 540 | 7.1 | 670 | 9.0 | 0.80 |
1 × 14 | 189 | 1700 | 8.9 | 2,300 | 12.0 | 0.74 |
1 × 9 | 299 | 5500 | 18.3 | 5,100 | 17.1 | 1.07 |
1 × 2 | 409 | 5000 | 12.2 | 5,800 | 14.3 | 0.86 |
1 × 10 | 533 | 5700 | 10.8 | 9,400 | 17.7 | 0.61 |
5 × 14 | 114 | 950 | 8.3 | 1,100 | 10.1 | 0.83 |
5 × 9 | 224 | 3700 | 16.6 | 3,500 | 15.8 | 1.05 |
5 × 2 | 334 | 4800 | 14.3 | 4,100 | 12.4 | 1.16 |
5 × 10 | 458 | 6000 | 13.2 | 5,900 | 12.8 | 1.03 |
14 × 9 | 110 | 1100 | 9.9 | 1,400 | 12.8 | 0.78 |
14 × 2 | 220 | 1600 | 7.3 | 2,000 | 9.3 | 0.79 |
14 × 10 | 344 | 2400 | 6.9 | 4,000 | 11.6 | 0.59 |
9 × 2 | 110 | 930 | 8.4 | 1,100 | 10.2 | 0.83 |
9 × 10 | 234 | 1900 | 8.2 | 2,400 | 10.3 | 0.80 |
2 × 10 | 124 | 480 | 3.9 | 750 | 6.0 | 0.64 |
↵a The mutation stated first is the one closer to the 5′ end of the gene.
↵b The distance between the mutation sites in base pairs.
↵c In type III crosses, the mutation closer to the 5′ end is in the h− strain; in type IV crosses, it is in the h+ strain.
↵d Prototrophic recombinants per million spores (one experiment per cross).
↵e Prototrophs per million divided by base pairs (distance between mutations).
↵f Prototrophs per million of the type III cross divided by ppm of the type IV cross.