TABLE 2

Conversion frequencies of ura4A mutations in one-point crosses

Conversionsc
MutationPositionaSequence changeCross
 typeb6:22:6Tetrads
 analyzedConversion (%)
13 562 C to AII241320817.8
 1 634 A to GI132421317.4
 5 709 G to TI 62021412.1
 6 793 G to CI10 7200 8.5
14 823 C to AI 81621711.1
 9 934 G deletionII14 5216 8.8
121001 G insertionI 415217 8.8
 21043 G to AI 8 8211 7.6
 41066 G to AII 4 8153 7.8
 31104 C to TI151222012.3
111163 22-bp repeatII13 2205 7.3
101167 C to TI 210204 5.9
  • a Numbering according to Grimm et al. (1988). First base of start codon is 534; first base of stop codon is 1326.

  • b Cross type I is h ura4A × h+ ura4A+; cross type II is h ura4A+ × h+ ura4A.

  • c In this tetrad run it was not possible to detect PMS. A conversion of 6:2 means three ura4A+ spore colonies (prototrophs) and one ura4A colony (auxotroph); 2:6 conversion tetrads have only one ura4A+ spore colony.