TABLE 1

Effect of timblind on bioluminescence rhythms emanating from per-luc and tim-luc transgenic flies in various genetic backgrounds

Genotypen% rhythmicτ (hr)Rel-ampPhase (hr)
LD
 plo48 9824.2 ± 0.00.25 ± 0.0118.4 ± 0.2
 timblind; plo31 9724.3 ± 0.10.35 ± 0.0217.1 ± 0.3
 NOG-luc3610024.3 ± 0.10.23 ± 0.0118.9 ± 0.2
 NOG-luc; timblind42 9824.2 ± 0.10.32 ± 0.0118.1 ± 0.2
 BG-luc7010024.4 ± 0.00.20 ± 0.0119.5 ± 0.1
 BG-luc; timblind7410024.1 ± 0.00.25 ± 0.0120.3 ± 0.2
 tim-luc4310024.3 ± 0.10.26 ± 0.0121.1 ± 0.2
 tim-luc; timblind47 9824.3 ± 0.10.34 ± 0.0220.5 ± 0.2
DD
 8.0-luc1310023.6 ± 0.20.42 ± 0.02NA
 timblind; 8.0-luc40 9523.7 ± 0.20.48 ± 0.01NA
 BG-luc21 8625.5 ± 0.30.49 ± 0.02NA
 BG-luc; timblind23 6525.4 ± 0.50.55 ± 0.02NA
  • Transgenic flies (all in a y w genetic background) were recorded for 5–7 days in 12 hr:12 hr LD cycles or for 2 days in LD and then released into DD at 25°. Data were analyzed using FFT-NLLS software (materials and methods) to determine “period” (τ), rel-amp, and the peak time of reporter expression (or “phase”, as the number of hours after lights on in the LD cycles). Note that all timblind reporter lines have weaker rhythms compared with their wild-type counterparts, indicated by relatively high relative-amplitude errors for the former, and show an earlier peak of luciferase expression in LD (except for BG-luc and 8.0-luc). Several independent insertion lines of the BG-luc transgenic type showed increased free-running periods of reporter-gene oscillations, as does the BG-luc60 type in this study (cf. Stanewsky et al. 1997a). NA, not applicable.