TABLE 2

Frequency of recombination between divergent direct repeats

Location within the U repeatb
Degree of divergenceaA zoneB zoneC zone
0 mismatches (0% divergence)Normalized average  1  1  1
No. of seedlings262262262
SDc  0.199  0.199  0.199
1 mismatch (0.162% divergence)Normalized average  0.284  0.386  0.349
No. of seedlings152156177
SDc  0.133  0.109  0.151
3 mismatches (0.485% divergence)Normalized average  0.510  0.206  0.233
No. of seedlings185145173
SDc  0.076  0.096  0.093
6 mismatches (0.971% divergence)Normalized average  0.132  0.226  0.186
No. of seedlings156158181
SDc  0.070  0.021  0.074
10 mismatches (1.618% divergence)Normalized average  0.153  0.225  0.355
No. of seedlings192160110
SDc  0.075  0.103  0.110
  • a The degree of divergence is expressed as the number of nucleotides that did not match (mismatches) or as the percentage of divergence [(number of mismatches) × 100/618] between the two 618-bp U repeats.

  • b The location within the U repeat is shown in detail in Figure 2.

  • c The standard deviation (SD) was calculated from the variation among the eight samples used in each treatment (mismatch-region combination). Recombination values, in each sample, were based on the average number of spots per seedling in ∼25 seedlings.

  • The frequency of recombination events, as estimated by the number of blue spots per seedling, was normal-ized relative to the identity (0 mismatches) construct.