TABLE 2

Repair junctions from spn-A mutants

Left enda
 acccagacCATGATGAAATAACATA
 tgggtctgRight end
 acccagac
 ATACAATAAAGTAGTACtgggtctg
Junctions with microhomologyb
acccagacCATGATGAcccagac
acccagacCATGATGAAATAACATGTTATTTCATCATGacccagac
acccagacCATGATGAAATAACATATGTTATTTCATCATGacccagac
acccagacCATGATGAAATAACACCTTATGTTATTTCATCATGacccagac
acccagacCATGATGTTATTTCATCATGacccagac
acccagacCATGATGAAATAACATGacccagac
acccagacCATGAcccagac
TGACCCAGACTGacccagac
acccagac…(165 nt)…AAATTTGTTATTTCATCATGacccagac
acccagac…(150 nt)…AAATGAATGTTATTTCATCATGacccagac
Junctions with T-nucleotidesc
acccagacCATGATGAAATAACATA[a]CATCATGacccagac
acccagacCATGATGA[g]CATCATGacccagac
acccagacCA[ag]ATGacccagac
acccagacCATGATGA[ac]CATCATGacccagac
acccagacCATGATGAAATAACATA[ac]ATGTTATTTCATCATGacccagac
acccagacCATGATGAAATAACAT[ttaacataac]ATGTTATTTCATCATGacccagac
acccagacCATGATGAAATAA[taataataatataa]TATGTTATTTCATCATGacccagac
acccagacCATGATGAAATAACAT[gtattacataac]ATGTTATTTCATCATGacccagac
acccagacCATGATGAAATA[tcatgaaatatcata]TCATCATGacccagac
  • a For the left and right ends, the lowercase represents the 8-bp genomic target site that is duplicated in the P{wa} insertion; the P-element sequence is given in uppercase. The overhangs remaining after P-element excision are shown at the top of the table.

  • b Underlined sequences in the center of the table represent the apparent microhomology between the left and right ends. The first sequence was observed eight times, the second three times, and the third twice. All other sequences were unique. The numbers in parentheses in the last two sequences under “Junctions with microhomology” indicate the length of sequence from repair synthesis.

  • c Inserted nucleotides are listed in lowercase in the center. Possible templates and templated insertions are underlined.