TABLE 1

Distribution of mutations fixed in haploid and diploid populations of S. cerevisiae after 100 generations in 16 μg/ml FLC

HaploidDiploid
MIC
MIC:a
 HaploidMIC:
 DiploidGeneticbMutationDiploidHaploidDiploidGeneticMutation
PDR1 N terminus
 6432PDR1G866A Arg289Ile326432cG844T Gly282Cys
 3216PDR1C893T Pro298Leud646464PDR1G906T Lys302Asn
 6464PDR1G924T Met308Ile326432PDR1G906T Lys302Asn
646464T926C Leu309Val
PDR1 C terminus
 6416T2215A Phe739Iled646464T2246C Phe749Ser
 6432T2215A Phe739Ile326432Not PDR3T2444C Phe815Ser
 6464PDR1T2246C Phe749Ser326432C2461T Arg821Leu
 6432C2258G Ser753Cys646464C2461A Arg821Ser
 6432C2250A Thr817Lys326432PDR1G3125A Gly1042Glu
 6464PDR1G2462A Arg821His
 6416PDR1G2620T Asp874Tyrd
 6432PDR1A2635G Tyr879Ala
PDR3 N terminus
 6416PDR3G758A Gly253Vald326432PDR3G844T Val282Phe
 3216Not PDR1G758A Gly253Vald
 6416PDR3G758A Gly253Vald
 6416PDR3G758A Gly253Vald
PDR3 C terminus
 6416PDR3G2120T Cys707Phed326432PDR3G2860T Val954Phe
 6416G2120T Cys707Phed326432PDR3G2860T Val954Phe
 6416PDR3G2120T Cys707Phed646464PDR3G2860T Val954Phe
 6432PDR3A2163C Lys721Asn646432PDR3G2860T Val954Phe
 6416Not PDR1G2381C Arg794Thrd326432PDR3G2860T Val954Phe
 6464PDR3T2444A Phe815Tyr326432PDR3G2860T Val954Phe
 6416PDR3A2483C Lys828Thr/326432PDR3G2860T Val954Phe
 T2489C Val830Alad646432PDR3G2860T Val954Phe
 6416PDR3T2839G Leu947Vald646432PDR3G2860T Val954Phe
 6432PDR3G2860T Val954Phe646464PDR3G2860T Val954Phe
 6432PDR3G2860T Val954Phe326432PDR3G2860T Val954Phe
646432PDR3G2860T Val954Phe
326432PDR3G2860T Val954Phe
646432PDR3G2860T Val954Phe
646464PDR3G2860T Val954Phe
326432PDR3G2860T Val954Phe
646464G2860T Val954Phe
ERG3
 25616Not PDR1,
   not PDR3C800A Thr267Asnd
Unidentified
 3216Not PDR1,
   not PDR3Unknownd326432PDR3
 6416Unknownd646432Not PDR1
 3216Unknownd
  • a For haploid populations, MIC was determined for the original culture and in a diploid formed from the mating of the original haploid with a FLC-sensitive haploid of compatible mating type. For diploid populations, MIC was determined for the original population, for haploid FLC-resistant, meiotic offspring, and for diploids formed from mating the FLC-resistant offspring with a FLC-sensitive haploid.

  • b Genetic tests for allelism were as described in the text. “not PDR1” and “not PDR3” mean that only one of the two tests for allelism was done and there was no match.

  • c The test was not done.

  • d These mutations were recessive.