TABLE 4

Summary of clinal variation of polymorphic sites at fw and 10 neutral loci

r2
LocusSiteFrequency  SlopeAll populationsIndiaSE AsiaEasternmost
fw 8340.07 9.79NS 0.821*
(25)10040.53 8.84  0.782***NS0.886* 0.812*
15040.49 10.46  0.857***0.929*0.886* 0.812*
 6870.49 10.46  0.857***0.929*0.886* 0.812*
 9690.49 10.46  0.857***0.929*0.886* 0.812*
10690.19 8.9  0.305*NSNS
18540.44 −10.57  0.693***0.987**NS
22920.06  −6.05NS0.589*
29610.44 −10.57  0.693***0.987**NS
39940.49 10.46  0.857***0.929*0.886* 0.812*
40230.05 −11.8NS0.948*
41060.49 10.46  0.857***0.929*0.886* 0.812*
1   40.07 −22.65NSNSNS 0.808*
(35)  100.14 6.81NSNSNS 0.850*
  140.19 5.74NSNSNS 0.825*
  530.14 −20.91  0.335*NSNSNS
2  280.05 21.56NS 0.890*
(63)  470.12 −23.67  0.283*NSNSNS
  480.10 −48.77  0.424*NSNS
  800.78 5.46NS0.933**NS
  830.88 10.27  0.290*0.857*NS
  890.02 −94.96  0.270*0.852*
  900.05  −5.48NS0.852*NS
  940.02 77.84  0.308*NS
3   30.31 5.35NS0.928*NSNS
(60)   80.01114.99  0.280*
   90.01104.11  0.251*
  110.15 3.96NS0.994*NSNS
  130.05 10.80NS0.840*NS
  180.01114.99  0.280*
  470.01114.99  0.280*
  520.81  −1.16NSNSNS 0.802*
  530.80  −2.78NSNSNS 0.802*
4  720.07 23.81NSNS 0.795*
(34)
5   20.18 −18.56NS0.936*NSNS
(27)  130.05 −57.94  0.343*NSNS
6   70.18 28.16  0.398*NSNSNS
(10)
7  100.10 8.36NSNSNS 0.821*
(19)  200.01−126.33  0.370**
8  80.03 −50.13  0.305*NS
(15)
9  220.91 18.28  0.454**NS 0.959**
(29)  250.33  −4.82NS0.931*NSNS
10   70.01−129.66  0.356*0.852*
(34)  210.25 12.62  0.315*0.943*NSNS
  230.86 4.74NS0.935*NSNS
  390.27 13.13  0.303*NSNSNS
  460.16 10.41NSNSNS 0.874*
  580.37 −10.48  0.313*NSNSNS
  • * P < 0.05;

  • ** P < 0.01;

  • *** P < 0.001.

  • The numbers of polymorphic sites analyzed for clinal variation (singletons were eliminated) at each locus are indicated in parentheses in column 1. Only sites displaying significant clinal variation in one or more subsets (see below) are shown. The frequency of individual sites is calculated for the entire pooled sample, on the basis of the derived state of the polymorphism as determined by the outgroup D. pallidosa. The slopes are computed from transformed data based on the entire pooled sample. Regressions (r2) of transformed allele frequencies on latitude were performed for all the populations combined, as well as the following subsets: India (KATH, BBS, PUR, and CH), SE Asia (MAN, CNX, BKK, and BOG), and Easternmost (KMJ, MNL, CEB, KK, BOG, and DAR). Polymorphic sites monomorphic or occurring only once in individual subsets are indicated by dashes. NS, no significant clinal variation.