TABLE 2

Distribution of parental and recombinant progeny for strains presented in Table 1

Single sporesaTetrads
Relevant genotypeParentalRecombinantRfcMPDTTNPD
URA3-LEU2
Wild type 363510090.21722.8607456 5
pms1Δ  539 1400.20620.97453 0
mms4Δ 2227 5050.18516.710251 0
mlh1Δ 3393 3990.10511.4486128 2
msh5Δ 5352 3220.0575.764376 1
mlh1Δ msh5Δ 5928.5 580.50.0899.8639120 5
mlh1Δ mms4Δ 2234  260.0120.51992 0
mms4Δ msh5Δ 1876  440.0231.0511 0
mlh1Δ mms4Δ msh5Δ 1739.5  50.50.0282.0492 0
   LEU2-LYS2
Wild type 338812560.27027.5496569 3
pms1Δ  504 1750.25826.86462 1
mms4Δ 2081 6510.23826.57775 1
mlh1Δ 3309 4380.12712.7459157 0
msh5Δ 5047 6270.11112.0562155 3
mlh1Δ msh5Δ 5672 8370.12916.2557199 8
mlh1Δ mms4Δ 2206  540.0242.519110 0
mms4Δ msh5Δ 1779 1410.0737.7448 0
mlh1Δ mms4Δ msh5Δ 1641.5 148.50.0839.84110 0
LYS2-ADE2
Wild type 4052 5920.12712.9803263 2
pms1Δ  591  880.13012.29631 0
mms4Δ 2447 2850.1049.512429 0
mlh1Δ 3517 2750.0736.953185 0
msh5Δ 5409 2650.0474.265961 0
mlh1Δ msh5Δ 6118 3910.0605.967984 1
mlh1Δ mms4Δ 2242  180.0081.21965 0
mms4Δ msh5Δ 1867  530.0283.8484 0
mlh1Δ mms4Δ msh5Δ 1745.5  44.50.0251.0501 0
ADE2-HIS3
Wild type 303316110.34737.734370916
pms1Δ  477 2020.29731.55768 2
mms4Δ 1923 8090.29630.75994 0
mlh1Δ 3104 6880.18119.6400211 5
msh5Δ 4797 8770.15518.7496215 9
mlh1Δ msh5Δ 539411150.17120.5495260 9
mlh1Δ mms4Δ 2193  670.0302.719011 0
mms4Δ msh5Δ 1779 1410.0734.8475 0
mlh1Δ mms4Δ msh5Δ 1611.5 178.50.1007.8438 0
URA3-LYS2
Wild type 281518290.39448.226475945
pms1Δ  390 2890.42652.82892 7
mms4Δ 1764 9680.35443.84998 6
mlh1Δ 2976 8160.21523.1351261 4
msh5Δ 4843 8310.14616.8513300 7
mlh1Δ msh5Δ 5248.51260.50.19426.048126023
mlh1Δ mms4Δ 2188  720.0323.018912 0
mms4Δ msh5Δ 1757 1630.0858.7439 0
mlh1Δ mms4Δ msh5Δ 1619.5 170.50.0959.84110 0
LYS2-HIS3
Wild type 282918150.39147.827874446
pms1Δ  451 2280.33639.84677 4
mms4Δ 1806 9260.33935.95398 2
mlh1Δ 2917 8750.23126.534426111
msh5Δ 463810360.18322.246524213
mlh1Δ msh5Δ 512713820.21226.243831115
mlh1Δ mms4Δ 2177  830.0373.718615 0
mms4Δ msh5Δ 1736 1840.0968.7439 0
mlh1Δ mms4Δ msh5Δ 1587.5 202.50.1138.8429 0
  • a In rare cases sectored spores were observed. They were assigned as half parental (0.5) and half recombinant (0.5).

  • Rf refers to the recombination frequency in single spores determined by parental/(parental + recombinant) and cM indicates the genetic distance in tetrads calculated using the formula of Perkins (1949): 50 × {TT + (6 × NPD)}/(PD + TT + NPD).