TABLE 1

Genetic map distances (cM) in wild type, mms4Δ, msh5Δ, and mlh1Δ strains

Relevant genotypeNo. analyzedURA3-LEU2LEU2-LYS2LYS2-ADE2ADE2-HIS3URA3-LYS2LYS2-HIS3
Tetradsa
Wild type106821.8–23.826.6–28.412.1–13.736.5–38.946.5–49.946.0–49.6
mms4Δ 15314.8–18.623.8–29.2 7.9–11.128.7–32.739.2–48.432.8–39.0
mlh1Δ 61610.3–12.511.8–13.6 6.2–7.618.2–21.021.8–24.424.7–28.3
msh5Δ 720 5.0–6.411.0–13.0 3.7–4.717.2–20.215.5–18.120.5–23.9
mlh1Δ msh5Δ 764 8.7–10.914.9–17.5 5.2–6.619.1–21.924.1–27.924.5–27.9
mlh1Δ mms4Δ 201 0.1–0.9 1.7–3.3 0.7–1.7 1.9–3.5 2.2–3.8 2.8–4.6
mms4Δ msh5Δ  52 0.1–2.0 5.2–10.2 2.0–5.6 2.8–6.8 6.1–11.3 6.1–11.3
mlh1Δ mms4Δ msh5Δ  51 0.6–3.4 7.0–12.6 0.1–2.0 5.3–10.3 7.0–12.6 6.1–11.5
Single sporesb
Wild type464420.6–23.025.8–28.411.8–13.833.3–36.138.0–40.837.7–40.5
mms4Δ273217.1–20.022.3–25.5 9.3–11.727.9–31.433.6–37.332.1–35.7
mlh1Δ3792 9.6–11.611.7–13.9 6.5–8.116.9–19.420.2–22.921.7–24.5
msh5Δ5674 5.1–6.310.3–11.9 4.1–5.314.5–16.413.8–15.617.3–19.3
mlh1Δ msh5Δ6509 8.3–9.712.1–13.7 5.5–6.616.2–18.118.4–20.420.3–22.3
mlh1Δ mms4Δ2260 0.8–1.7 1.8–3.1 0.5–1.3 2.3–3.8 2.5–4.0 3.0–4.6
mms4Δ msh5Δ1920 1.7–3.1 6.2–8.6 2.1–3.6 6.2–8.6 7.3–9.9 8.3–11.0
mlh1Δ mms4Δ msh5Δ1790 2.1–3.7 7.1–9.7 1.8–3.3 8.6–11.4 8.2–11.0 9.9–12.9
  • a Intervals correspond to the genetic distance calculated from tetrads ±1 standard error. Standard error was calculated using the Stahl Laboratory Online Tools website (http://groik.com/stahl/).

  • b Data shown as 95% confidence intervals around the recombination frequency determined from single spores, calculated using the VassarStats website (http://faculty.vassar.edu/lowry/VassarStats.html). To facilitate comparisons to the tetrad data, recombination frequencies obtained from single-spore data were multiplied by 100 to yield genetic map distances (in centimorgans).

  • All mutants are isogenic derivatives of EAY1108/EAY1112 (materials and methods).