TABLE 3

Tissue-specific extrachromosomal expression of the wild-type tpk-1 gene fused to gfp and expressed in a tpk-1 mutant background

Genotype (extrachromosomal arrays)Brood size (progeny) (n = 5)Defecation
 cycle length
 (sec) (n = 20)Pharyngeal
 pumping
 (pumps/min)
 (n = 20)Postembryonic
 development
 (hr) (n = 50)
Wild type (N2)305 ± 16 55.6 ± 3.8248 ± 16.847.7 ± 3.5
tpk-1 76 ± 7 115 ± 23.6140 ± 23.860.5 ± 4.5
[myo-3::tpk-1:gfp] 25 ng/μl, line 1a253 ± 11 59.3 ± 4.1243 ± 14.249.2 ± 3.2
[myo-2::tpk-1:gfp] 25 ng/μl, line 1a167 ± 21  65 ± 5.9245 ± 1249.1 ± 3.4
[myo-2::tpk-1:gfp] 5 ng/μl, line 1a176 ± 10  77 ± 6.2207 ± 11.256.1 ± 3.5
[myo-2::tpk-1:gfp] 5 ng/μl, line 2a191 ± 40 64.9 ± 5.9215 ± 1252.4 ± 3.3
[myo-2::tpk-1:gfp] 5 ng/μl, line 3a167 ± 46 83.5 ± 20.3217 ± 21.751.4 ± 3.4
[myo-2::tpk-1:gfp] 1 ng/μl, line 1 84 ± 10 101 ± 20141 ± 19.758.2 ± 4.7
[myo-2::tpk-1:gfp] 1 ng/μl, line 2aND 73.4 ± 3.8208 ± 12.6ND
[myo-2::tpk-1:gfp] 1 ng/μl, line 3 80 ± 16114.3 ± 18.5142 ± 18.259.9 ± 4.9
  • a Lines whose individuals have a wild-type appearance.