TABLE 2

Effect of thiamine and TPP on recovery from larval arrest

Duration of development to adulthood after L1 arrest (days)
Wild typetpk-1
Duration of
 arrest (days)aTTPPM9 bufferTTPPM9 buffer
1222333
2222333–4
3222334
42–32–333–43–45b
52–32–333–43–46–7b
  • a L1 larvae were arrested on empty plates supplemented with 200 μl thiamine (25 mm), TPP (25 mm), or M9 buffer. Worms were transferred to plates with bacteria at the indicated day and the duration of development to adulthood was determined.

  • b Some worms had already died; others appeared developmentally arrested.