TABLE 3

atx-2 promotes the oocyte fate

GenotypeTemp (°)% sperm +
 oocytes% Mog% Fog% otheran
atx-2(RNAi) feedingb205644  0 0424
atx-2(RNAi) injection202377  0 0 75
atx-2(RNAi) feedingb252872  0 0186
fog-2(q71 or oz40)NA 0 0100c 0
atx-2(RNAi)d208218  0 0 50
fog-2(oz40); atx-2(RNAi)d2056 6 2512.5 16
fog-2(q71); atx-2(RNAi)d206238  0 0 16
fog-2 mock RNAid20 0 0100 0 40
  • a No gametes were produced.

  • b Data are the average of four or more independent feeding experiments; % Mog germlines ranged from 18 to 83% at 20° and 50 to 97% at 25°.

  • c Schedl and Kimble (1988); Clifford et al. (2000).

  • d Wild type and fog-2 were treated in parallel, with wild type serving as a control for efficacy of the RNAi in this specific set of experiments. Mock RNAi was done by feeding HT115 cells containing the L4440 vector to fog-2 mutants to make sure that this treatment per se did not suppress the Fog phenotype.

  • n, number of gonad arms examined; NA, not applicable.