TABLE 1

Genetic and molecular resources that can be applied to wheat genome sequencing analysis

TargetGenetic mapsPhysical genome resources
Hexaploid wheat, T. aestivum L. cv.
   Chinese SpringMore than 4700 markers have been placed
   into 20 genetic maps of wheat and its
   relatives, and all the mapping data are
   deposited in the GrainGenes database
   (http://wheat.pw.usda.gov/ggpages/
   mapsframe.html)A total of 500,000 ESTs are recorded and
   ∼6000 unigenes are assigned to 159
   bins across the 21 wheat chromosomes
   on the basis of deletion and ditelosomic
   stocks (http://wheat.pw.usda.gov/nsf)
Genetic stocks in Chinese Spring
   wheat, their chromosome
   constitution and number
   of lines available:On the basis of these maps, a composite
   linkage map has been compiled.
   Markers on the map are linked
   electronically to the Catalog of
   Gene Symbols for Wheat
   (http://wheat.pw.usda.gov/
   ggpages/wgc)A total of 200,000 BAC clones are arranged
   as contigs across the entire D-genome
   donor of hexaploid wheat. More than
   1000 ESTs are assigned to BAC clones
   (http://wheatDB.ucdavis.edu)
Ditelosomic (20′′ + t′′), 21Individual genetic maps are available in
   cMAP format through an internet
   interface276,480 BAC clones of T. monococcum
   (Lijavetzky et al. 1999)
Double-ditelosomic
   (20′′ + tS′′ + tL′′), 20516,096 BAC clones of durum wheat
   (Cenci et al. 2003)
Ditelo-monotelosomic
   (20′′ + t′′ + t'), 411,200,000 BAC clones from cv. Chinese
   Spring (Allouis et al. 2003)
Double monosomic
   (19′′ + 1′ + 1′), 2087,168 BAC clones available from 3B;
   87,168 BAC clones from 1D, 4D + 6D
   combined, and 65,280 BAC clones from
   1BS (Safar et al. 2004)
Monosomics (20′′ + 1′′), 21.1,000,000 BAC clones from cv. Renan,
   INRA-Genoplante Resources (B.
   Chaloub, personal communication)
Monoisosomic (20′′ + 1′), 7
Trisomics (20′′ + 1′′′), 21
Nullisomic-tetrasomic
   (20′′ + 1′′′′), 38
Monosomic-tetrasomic
   (20′′ + 1′ + 1′′′′), 4
Nullisomic (20′′), 8
Single chromosome substitution
   lines, 184
   (http://www.ksu.edu/wgrc)
  • The listed BAC libraries are public resources and free of IPR.