TABLE 3

Pairing of chromosomes investigated by hybridization with subtelomeric vs. interstitial probes

Background
 labeling (%)
StrainProbe chromosome
 positionHarvesting time (hr)1 dot (%)2 dots (%)>2 dots0 dots% pairing after
 subtracting
 backgroundN
spo11Δ  Subtelomeric54046 9 547415
spo11Δndt80Δ  Subtelomeric54540 8 753224
spo11Δndt80Δ  Subtelomeric84640 6 853181
Wild type  Subtelomeric54737 9 756180
spo11Δ  Interstitial52364 4 926287
spo11Δndt80Δ  Interstitial52857 7 933172
spo11Δndt80Δ  Interstitial83059 3 734174
Wild type  Interstitial54830121062129
  • The percentage of mutant and wild-type cells undergoing homologous chromosome pairing and nonpairing in FISH experiments with subtelomeric (ATCC no. 70884) and interstitial (ATCC no. 71216) probes. Both mutant and wild-type cells were analyzed at pachytene. We judged pairing to have taken place if the two FISH signals were separated by <0.7 μm.