TABLE 1

Recombination in the left arm of chromosome VII

Setade5,7-URA3URA3-KANKAN-lys5lys5-NATNAT-HPHHPH-cyh2cyh2-crl3crl3-trp5trp5-leu1
A. Crossovers in nine intervalsa
IPD:TT:NPD1082:827:141138:756:151234:780:51478:557:61724:332:01805:240:6846:1168:401201:843:131520:539:4
cM23.7 ± 0.822.2 ± 0.820.1 ± 0.614.5 ± 0.68.1 ± 0.4 6.7 ± 0.534.3 ± 1.022.4 ± 0.713.7 ± 0.6
IIPD:TT:NPD 366:980:77 866:539:11 882:526:111193:234:21160:269:4492:887:50 842:568:13 941:481:6
cM50.7 ± 1.721.4 ± 0.920.9 ± 0.98.6 ± 0.610.2 ± 0.741.5 ± 1.422.7 ± 1.018.1 ± 0.8
kb4958.3494513.129.798.23527.8
Avg.kb/cM, 2.1 2.6 2.4 2.5 1.6 3.5 2.6 1.6 1.7
ade5,7URA3 (set I)KANlys5NATmet13HPHcyh2crl3trp5leu1
B. Conversion at eleven markersb
3:1  7/3  113 25/10 14/14  1/8 104/65 3/4 7/2 4/11 6/9 2/4
1:3  7/10   18  6/5 10/5  7/1  79/65 7/6 6/5 5/2 2/7 1/3
Frequency  0.008  0.063 0.013 0.012  0.005 0.089 0.006 0.006 0.006 0.007 0.003
Other (no.)  4:0 (1)  4:0 (6) 4:0 (1)  0:4 (2) 0:4 (2) 0:4 (1) 0:4 (1) 4:0 (2) 4:0 (2) 4:0 (1)
  0:4 (1) 0:4 (2) 4:0 (2)
  • a To determine the map length in each elementary interval along chromosome VII, 2076 four-spore tetrads (set I) and 1451 four-spore tetrads (set II) were scored. The centromere is to the right. Those tetrads exhibiting non-Mendelian segregation of either marker defining a given interval are not included. Map distances and standard errors in centimorgans (cM) were calculated with the aid of Stahl Lab Online Tools (http://groik.com/stahl/), on the basis of the formula cM = 100 × (TT + 6NPD)/2(PD + NPD + TT), which assumes that all intervals are subject to no more than two crossover events. Physical distances were determined from data in the Saccharomyces Genome Database (http://genome-www.stanford.edu/Saccharomyces/).

  • b Non-Mendelian segregations observed in the 2076 analyzed tetrads of set I and the 1451 tetrads of set II (entered as set I/set II). For Frequency and for Other, data from the two sets are combined.