TABLE 2

Summation of subgenomic microsatellite-enriched libraries from Xiphophorus

CA library
 (%)ATG library
 (%)TACA library
 (%)TAGA library
 (%)Total (%)
Total clonesa850 459 280 4852074
Uniqueb418 (49) 138 (30)  50 (18) 122 (25) 728 (35)
Bad sequencec 49 (6)  24 (5)  12 (4)  40 (8) 125 (6)
No repeatd 42 (5) 111 (24)  88 (31)  58 (12) 299 (14)
Duplicatee204 (24) 143 (32) 105 (38) 222 (46) 674 (33)
Too close to vectorf137 (16)  43 (9)  25 (9)  43 (9) 248 (12)
  • a The total number of clones that were isolated from the libraries.

  • b The number of clones with unique sequences when compared to all other clones.

  • c Plasmid clones that had been sequenced, but yielded too many ambiguities.

  • d Plasmids that did not show obvious repeats of the desired type (CA, ATG, etc.)

  • e Nonunique clones (see footnote a, above).

  • f Insufficient plasmid-insert nucleotide sequence information available for primer design.