TABLE 1

Selective gene mapping strategy in Xiphophorus

Gene	Forward primer	Reverse primer	Polymorphism  type	Restriction  endonuclease
APE	aggcaagaagacagatgatgggtc	tagaagtttgggaactcggctgtg	PCR/restriction	HaeIII
CDKN2X	taaaccagaacaactaagtgg	ctgtattgctcttcgtcca	PCR/size	None
ERCC2	agaacaacgtagacacgctgc	ggcagcatccgtctcctt	PCR/restriction	TaqI
FEN1	tagcaagtgggtaaaccaggctaac	tagatgcacatggaggcatcaatagcaat	PCR/restriction	CfoI
FoxO5	ctctcaccgatgatctactccagtc	gatggaggtgtcaatttgcatggaggatgttc	PCR/restriction	AluI
LIG1	gggcctgatggtgaagactct	tgtgtcgcccacaccgt	PCR/restriction	CfoI
NF1	gcttgctgtggaacaaccagg	tcgcctgccaactgcctt	PCR/restriction	HinfI
POLB	gcatccaccattgccaagtatcct	ttccagtttgtaagaactcatcgatcttttctg	PCR/restriction	AluI
RAB27	gcactaaggcagacatcagag	ggttcagagccaggggcccca	PCR/restriction	TaqI
TP53	cttcaggtcaggatttcagtcgc	cccacctccagtcctcttaactct	PCR/restriction	BclI
UNG	taggtcaagatccatatcatggtc	gatgctgaaagccgtcga	PCR/size	None