TABLE 1

Yeast strains and plasmids

StrainGenotypePlasmidStrain backgroundSource/parent strain
YH23MATα Δ ura3-167 his3Δ200 leu2Δ1 trp1Δ1pX3  GRF167Jef Boeke/YH8
JB721MATa his3Δ200 ura3-167
JKc125MATα ura3-167 his3Δ200 leu2Δ1 trp1Δ1 sir4-119pGTy1H3mhis3AI  GRF167This study
YPH645MATa ura3-52 his3Δ200none  S288CPhilip Heiter
JKc1005MATα ura3-167 his3Δ200 leu2Δ1 sir4-119pGTy1H3mhis3AI  GRF167This study
JKc1015MATα ura3-167 his3Δ200 leu2Δ1pGTy1H3mhis3AI  GRF167This study
JKc1065MATa his3Δ200 leu2Δ1 met15Δ0 trp1Δ63
   ura3-167 RDN1::Ty1-MET15 sir3Δ::kanMX4
   sir4Δ::HIS3pX3  GRF167Jeff Smith/JS331 ×
   JS3A-89
JKc1066MATα his3Δ200 leu2Δ1 met15Δ0 trp1Δ63
   ura3-167 RDN1::Ty1-MET15 sir2Δ::HIS3pX3  GRF167Jeff Smith/JS218 A, B
JKc1067MATα his3Δ200 leu2Δ1 met15Δ0 trp1Δ63
   ura3-167 RDN1::Ty1-MET15 sir3Δ::HIS3pX3  GRF167Jeff Smith/JS244 A, B
JKc1068MATa his3Δ200 leu2Δ1 met15Δ0 trp1Δ63
   ura3-167 RDN1::Ty1-MET15 sir2Δ::kanMX4
   sir4Δ::HIS3pX3  GRF167Jeff Smith/JS346
JKc1072MATα ade2-1 can1-100 his 3-11,15 leu2-3 trp1-1
   ura3-1pGTy1H3mhis3AI  W303Alan Myers/W303-1B
JKc1075W303-1B ADE2 lys2Δ sir4::LEU2pGTy1H3mhis3AI  W303Jasper Rine/ JRY4582
JKc1159JKc1015 fus3::kanMX4pGTy1H3mhis3AI  GRF167This study
JKc1160JKc1005 fus3::kanMX4pGTy1H3mhis3AI  GRF167This study
JKc1161JKc1015 spl2::kanMX4pGTy1H3mhis3AI  GRF167This study
JKc1162JKc1005 spl2::kanMX4pGTy1H3mhis3AI  GRF167This study
JKc1163MATα his3Δ200 leu2Δ1 met15Δ0 trp1Δ63
   ura3-167 RDN1::Ty1-MET15pX3  GRF167Jeff Smith/JS209
JKc1167JKc1005 rad52::LEU2pGTy1H3mhis3AI  GRF167This study
JKc1168JKc1015 rad52::LEU2pGTy1H3mhis3AI  GRF167This study
JKc1190MATα his3Δ200 leu2Δ1 met15Δ0 trp1Δ63
   ura3-167 RDN1::Ty1-MET15 sir4Δ::HIS3pX3  GRF167Jeff Smith/JS329
JKc1195JKc1005pJEF1105  GRF167This study
JKc1196JKc1015pJEF1105  GRF167This study
JKc1223JKc1005 hmr::LEU2pGTy1H3mhis3AI  GRF167This study
JKc1241JKc1015 hmr::kanMX4pGTy1H3mhis3AI  GRF167This study
Plasmid nameFeatures  Source
pX3TRP1-marked Gal-Ty1, 2μ URA3
pGTy1H3mhis3AIhis3mAI-marked Gal-Ty1, 2μ URA3Curcio and Garfinkel (1991b)
pSM20rad52::LEU2L. Prakash and S. Prakash
pJEF1105neo-marked Gal-Ty1, 2μ URA3Boeke et al. (1988)
pCS1FUS3, LEU2This study
pJK592LEU2 HIS3 CENThis study