TABLE 2

Features of normalized and subtracted cDNA libraries

Normalized/subtracted
 library designationParent libraryMethod for preparing
 single-stranded DNAaDriver cDNAC0t value
 (sec-mole/liter)Parent
 library titerEnriched
 library titer
TA001E2NTA001E1Xb2PCR-amplified DNAs from library TA001E1X  51.2 × 1053.5 × 107
TA006E2NTA006E1X1PCR-amplified DNAs from library TA006E1X  2.51.1 × 1062.4 × 109
TA006E3NTA006E1X2PCR-amplified DNAs from library TA006E1X  53.5 × 1054.1 × 107
TA008E3NTA008E1X2PCR-amplified DNAs from library TA008E1X  51.9 × 1079.0 × 109
TA005E2STA005E1X2Pooled equal amount of PCR-amplified DNAs from
   nonstressed seedling shoot library TA006E1X
   and seedling root TA008E1X1004.0 × 1048.1 × 106
TA007E2STA007E1X2Pooled equal amount of PCR-amplified DNAs from
   nonstressed seedling shoot library TA006E1X
   and seedling root TA008E1X1002.4 × 1044.0 × 106
TA007E3STA007E1X2Pooled equal amount of PCR-amplified DNAs from
   nonstressed seedling shoot library TA006E1X
   and seedling root TA008E1X 505.0 × 1045.5 × 105
TA027E1STA027E1X21st stranded cDNAs from non-stressed tissues1008.3 × 1062.4 × 105
  • a The two methods are described in Preparation of single-stranded phagemid DNA for normalization in materials and methods.

  • b Not included here is an additional subtracted library, TA001E1S, which was a collection of ESTs derived from TA001E1X identified by a different method (see materials and methods).